【問題】Degenerate primers ?推薦回答

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DegePrime, a Program for Degenerate Primer Design for Broad ...。

Such methods typically are based on PCR amplification of rRNA gene sequences using broad-taxonomic-range PCR primers. In these analyses, the use of optimal ...: tw | tw。

Development of a Semiquantitative Degenerate Real-Time PCR ...。

Several degenerate primers were designed and tested in real-time PCR experiments with the CoA transferase genes of R. hominis A2-183, A. caccae L1-92, E. hallii ...。

MDC-Analyzer: A novel degenerate primer design tool for the ...。

2018年4月3日 · By initially defining an objective function, the possible optimal degenerate PCR primer profiles could be automatically explored using the ...。

PCR effects of melting temperature adjustment of individual primers ...。

2019年3月4日 · One little noticed feature of such degenerate primer sets is the potential for a wide range of melting temperatures between the primer ...。

Accumulating Variation at Conserved Sites in Potyvirus Genomes Is ...。

2008年2月13日 · Unknown and foreign viruses can be detected using degenerate primers targeted at conserved sites in the known viral gene sequences.。

Polymerase Chain Reaction Cycle Sequencing with Degenerate ...。

Among the many techniques of cloning new genes, one approach involves degenerate primers (1–7). The approach usually requires three steps:1.: 。

Optimizing PCR primers targeting the bacterial 16S ribosomal RNA ...。

2018年9月29日 · Table 1 Example of the mapping from a pair of degenerate primers to a ... Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL.。

Multiplex degenerate PCR coupled with an oligo sorbent array for ...。

Nine HERV families were selected and amplification primers and capture ... The amplification efficiency and specificity of each degenerate primer pair was ...。

Plant Pathogen Detection and Disease Diagnosis。

Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. ... Xie, G.L. and Mew, T.W. 1998.。

(PDF) Targeting polyketide synthase gene pool within actinomycetes。

Keywords Actinomycetes Æ Degenerate primers Æ. Stepdown PCR Æ Polyketide synthases. Introduction ... of two distinctive sequences in strain NP13 (NP13 PKS1.


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